Improved Metabolite Isolation from Samples of Biological Origin Using Liquid Chromatography with Sub-2-Micron Particle Columns Application

In recent years, a sense of urgency has been placed on increasing the rate at which drugs are brought to market. [1] Therefore, every aspect of analysis should be achieved at an increased rate as well, without sacrificing the quality of the analysis. The recent adoption of small sub-2-micron particle columns has enabled faster and higher resolution liquid chromatography. When isolating metabolites from complex matrices such as plasma and urine, it is imperative that if the rate of sample isolation is to be increased, the overall purity or recovery of the collected metabolites must not be compromised. The advantages of small-particle columns in the application of isolating low-level metabolites are demonstrated.